An automated method for the determination of serum calcium with glyoxal bis (2-hydroxyanil).
نویسندگان
چکیده
1. 0.060% (w/v) Glyoxal bis (2-/iydroxyanil) solution (GBHA) C,4H12N202 (Fisher Scientific Co.) 600 mg. GBHA is dissolved in 1000 ml. absolute methanol, and stored in an amber bottle. This solution is stable for at least 1 month after preparation. 2. Buffer solution (pH 12.6) 10 gm. sodium hydroxide and 10 gm. sodium tetraborate (Na2B407. 1
منابع مشابه
Determination of serum calcium by glyoxal bis (2-hydroxyanil) chelation.
A simple micro (0.1 ml.) colorimetric method is described for the determination of serum calcium. This method employs the differential chelating characteristic of glyoxyl bis (2-hydroxyanil) in the presence of other divalent cations. The serum sample is placed in a colorimetric tube followed by additions of water, glyoxyl bis (2-hydroxyanil) reagent, and alkaline carbonate color developer. Chlo...
متن کاملDirect photometric analysis of serum calcium with glyoxal bis (2-hydroxyanil).
A spectrophotometric method for the direct determination of microgram quantities of calcium in serum is presented. In this procedure, 0.02 ml. of serum is interacted with buffered glyoxal bis (2-hydroxyanil) solution (GBHA) at pH 12.6. The resultant red calcium-GBHA complex is diluted in methanol-acetone-water solution, and the absorbance determined at 520 m . The effects of pH, temperature, CB...
متن کاملRapid Determination of Calcium in Milk and Water Samples by Reflectance Spectroscopy
Reflectance spectroscopy (RS) can be used as a rapid and sensitive method for the quantitative determination of low amounts of calcium. In this analytical technique, the analyte in complex samples is extracted onto a solid sorbent matrix loaded with glyoxal bis (2-hydroxyanil (GBHA) and then quantified directly on the sorbent surface. The measurements were carried out at a wavelength of 566.1 n...
متن کاملAn automated method for serum calcium utilizing ethylene diamine tetraacetic acid.
A FUNDAMENTAL DIFFICULTY in estimating calcium in serum by photometry arises from the fact that the normal range is narrow relative to the concentration of calcium. In the cases of sodium and chloride which also possess narrow normal ranges, the automation problem has been approached in two different ways. For sodium the Technicon Flame Photometer incorporates an electrical range expander. In t...
متن کاملDetermination of diethyllead in the urine by flameless atomic absorption spectrometry.
A method for the determination of diethyllead in urine by flameless atomic absorption spectrometry after chelation with glyoxal-bis (2-hydroxyanil) and extraction of the formed complex with methyl isobutyl ketone is described. The method is specific in relation to both triethyllead and inorganic lead. The limit of detection was 3.2 micrograms Pb/l and the relative standard deviation in the conc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 13 6 شماره
صفحات -
تاریخ انتشار 1967